HomeIngredientEvaluation of the mechanism of action of rosemary volatile oil in the treatment of Alzheimer’s disease using gas chromatography-mass spectrometry analysis and network pharmacology

Evaluation of the mechanism of action of rosemary volatile oil in the treatment of Alzheimer’s disease using gas chromatography-mass spectrometry analysis and network pharmacology

October 6, 2022

Comb Chem High Throughput Screen. 2022 Sep 30. doi: 10.2174/1386207325666220930091758. Online ahead of print.

ABSTRACT

Objective The aim of our study was to investigate the active components and mechanism of action of rosemary volatile oil for treating Alzheimer’s disease (AD) using network pharmacology. Methods We obtained the constituents of the rosemary volatile oil by searching Chinese herbal systemic pharmacological databases and analytical platforms, and we constructed the constituent-target networks by predicting and screening the action targets of the rosemary volatile oil constituents using SwissTargetPrediction, metaTarFisher, and Pubchem. We obtained the AD-related targets using the Genecards, OMIM, and DisGeNET databases, and we constructed the protein-protein interaction networks (PPI) using the STRING database in Venny 2.1.0 graph to screen the cross-targets by screening the core-acting targets. Cytoscape 3.8.2 software was used to construct a component-target-pathway network to screen the potential active components of the rosemary volatile oil for the treatment of AD and to predict the mechanism of action of the rosemary volatile oil for the treatment of AD in combination with existing pharmacological studies. We performed a gene ontology (GO) biological process and a Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the targets of the rosemary volatile oil for the treatment of AD using R language and molecular docking using Discovery Studio 4.0 software to validate their biological activities. Results A network constructed using gas chromatography-mass spectrometry (GC-MS) analysis identified 26 potential active ingredients in the rosemary volatile oil. We retrieved a total of 10762 AD targets from Genecards and other used databases. Our GO enrichment analysis yielded 39 entries (P < 0.05), including 14 entries for biological processes, five entries for cellular composition, and 20 entries for molecular function. A total of 14 entries (P < 0.05) were then enriched in the KEGG pathway that primarily involved the IL-17 signaling pathway and the AGE-RAGE pathway. Conclusion The active components of rosemary volatile oil had good inhibition of the inflammatory response. This study provides a reference and guidance for the in-depth study of rosemary volatile oil for the treatment of AD.

PMID:36200249 | DOI:10.2174/1386207325666220930091758

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About The Author

Patrick Blanchard MD

Meet Dr. Blanchard Dr. Blanchard’s medical practice is an embodiment of Integrative health which brings conventional and complementary approaches together in a coordinated way. Conveniently located in sunny Florida and over the internet with ValiseMD’s secure HIPPA compliant telXmed servers. Since 1994, patients with a wide range of challenging medical problems have achieved optimum health using the best of natural medicine, judiciously combined with high-tech innovations. Breakthroughs are often achieved even after patients have consulted mainstream specialists and holistic practitioners. Dr. Blanchard is founder and CEO of ValiseMD, Inc. He is board certified in Family Medicine since 1994 and awarded Fellow of the American Academy of Family Physicians in 2001. He received the ‘Teacher of the Year’ award from the University of Kansas School of Medicine at completion of his residency in Family Medicine. He completed a fellowship at Wake Forest University in the field of vascular neurosonology. He holds a medical patent in the field of Gastroenterology. He holds an unrestricted license to practice medicine and surgery in Florida. He started his medical career as a Emergency Medical Technician, then as a Paramedic and later a Medical Doctor.