HomeIngredientSynergistic effect of curcumin and resveratrol on the prevention of contrast-induced nephropathy by suppressing inflammation via regulating signaling pathways of microRNA-17/TXNIP/NRLP3 and microRNA-30c/FOXO3/NRLP3

Synergistic effect of curcumin and resveratrol on the prevention of contrast-induced nephropathy by suppressing inflammation via regulating signaling pathways of microRNA-17/TXNIP/NRLP3 and microRNA-30c/FOXO3/NRLP3

February 21, 2022

Biotechnol Appl Biochem. 2022 Feb 20. doi: 10.1002/bab.2333. Online ahead of print.

ABSTRACT

In this study, we aimed to explore the molecular mechanism underlying the effect of resveratrol (RES) and curcumin (CUR) on the treatment of CIN. And we also investigated the potential additive interactions between RES and CUR. Rat models were established as a Sham group, a contrast induced nephropathy (CIN) group, a CIN+RES group, a CIN+CUR group and a CIN+RES+CUR group. ELISA was performed to observe the expression levels of serum creatinine, blood urea nitrogen (BUN) and neutrophil gelatinase-associated lipocalin (NGAL) in the rats. H&E staining was performed to measure renal dysfunction of each rat group. Real-time PCR, Western blot, computational analysis and luciferase assays were used to establish the signaling pathways responsible for the effect of RES and CUR on CIN. The levels of serum creatinine, BUN and serum NGAL were significantly increased in CIN rats, which were recovered by the administration of RES and/or CUR. The high injury score in the CIN group was preserved by the administration of RES and CUR. The expression of both miR-17 and miR-30c was markedly decreased while the expression of thioredoxin-interacting protein (TXNIP), forkhead box O3 (FOXO3), NLR family pyrin domain containing 3 (NRLP3), interleukin-1 beta (IL-1β) and cleaved caspase-3 was all markedly increased in the CIN group. RES or CUR treatment mitigated the above dysregulation in CIN rats, and the combined administration of RES+CUR exhibited a more impressive effect. TXNIP was a target gene of miR-17, while FOXO3 was a target gene of miR-30c. The combined administration of RES and CUR additively reduced the expression of NLRP3, and attenuated renal injury and inflammation in CIN treatment. This article is protected by copyright. All rights reserved.

PMID:35184332 | DOI:10.1002/bab.2333

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About The Author

Patrick Blanchard MD

Meet Dr. Blanchard Dr. Blanchard’s medical practice is an embodiment of Integrative health which brings conventional and complementary approaches together in a coordinated way. Conveniently located in sunny Florida and over the internet with ValiseMD’s secure HIPPA compliant telXmed servers. Since 1994, patients with a wide range of challenging medical problems have achieved optimum health using the best of natural medicine, judiciously combined with high-tech innovations. Breakthroughs are often achieved even after patients have consulted mainstream specialists and holistic practitioners. Dr. Blanchard is founder and CEO of ValiseMD, Inc. He is board certified in Family Medicine since 1994 and awarded Fellow of the American Academy of Family Physicians in 2001. He received the ‘Teacher of the Year’ award from the University of Kansas School of Medicine at completion of his residency in Family Medicine. He completed a fellowship at Wake Forest University in the field of vascular neurosonology. He holds a medical patent in the field of Gastroenterology. He holds an unrestricted license to practice medicine and surgery in Florida. He started his medical career as a Emergency Medical Technician, then as a Paramedic and later a Medical Doctor.