HomeCannabis MedicineSimple and fast quantification of cannabinoids in animal feeds by liquid chromatography-tandem mass spectrometry

Simple and fast quantification of cannabinoids in animal feeds by liquid chromatography-tandem mass spectrometry

April 20, 2023

J Vet Diagn Invest. 2023 Apr 17:10406387231169290. doi: 10.1177/10406387231169290. Online ahead of print.

ABSTRACT

There is growing interest in using hemp materials as animal feed ingredients, which may raise safety concerns because of the potential transfer of active cannabinoids to the resultant products of animal origin. Hence, the detection and identification of cannabinoids in feeds would be useful. We developed a simple, fast, and sensitive method for simultaneous quantification of 4 major cannabinoids in animal feeds by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We used a simple solvent extraction and dilution approach to extract cannabinoids from the feed matrix. We validated the method in 2 types of cattle feeds with acceptable intra-day and inter-day accuracy (87.5-116%) and precision (< 15%). The limit of detection was 0.05 µg/g, and the limit of quantification was 0.1 µg/g. Furthermore, the method was able to identify and quantify cannabinoids in cattle feeds mixed with hempseed cake as well as in several different hempseed materials, demonstrating its potential in veterinary laboratory applications.

PMID:37067036 | DOI:10.1177/10406387231169290

Related Posts

About The Author

Patrick Blanchard MD

Meet Dr. Blanchard Dr. Blanchard’s medical practice is an embodiment of Integrative health which brings conventional and complementary approaches together in a coordinated way. Conveniently located in sunny Florida and over the internet with ValiseMD’s secure HIPPA compliant telXmed servers. Since 1994, patients with a wide range of challenging medical problems have achieved optimum health using the best of natural medicine, judiciously combined with high-tech innovations. Breakthroughs are often achieved even after patients have consulted mainstream specialists and holistic practitioners. Dr. Blanchard is founder and CEO of ValiseMD, Inc. He is board certified in Family Medicine since 1994 and awarded Fellow of the American Academy of Family Physicians in 2001. He received the ‘Teacher of the Year’ award from the University of Kansas School of Medicine at completion of his residency in Family Medicine. He completed a fellowship at Wake Forest University in the field of vascular neurosonology. He holds a medical patent in the field of Gastroenterology. He holds an unrestricted license to practice medicine and surgery in Florida. He started his medical career as a Emergency Medical Technician, then as a Paramedic and later a Medical Doctor.