Selenium nanoparticles and omega-3 fatty acid enhanced thermal tolerance in fish against arsenic and high temperature
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Nov;261:109447. doi: 10.1016/j.cbpc.2022.109447. Epub 2022 Aug 24.
ABSTRACT
The aquatic ecosystem is prone to global climate change and pollution affecting aquatic animals, including fish. In light of the above, we experimented with delineate the role of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) with selenium nanoparticles (Se-NPs) to enhance the thermal tolerance in Pangasianodon hypophthalmus reared under control or concurrent exposure to high temperature and arsenic (As + T) for 112 days. Se-NPs were synthesized using the green approach. Four experimental diets viz. EPA + DHA at 0.2, 0.4 and 0.6 % along with Se-NPs at 0.2 mg kg-1 diet were formulated and prepared. End of the experiment (112 days), the thermal tolerance viz. CTmin (critical thermal minima) CTmax (critical thermal maxima), LTmin (lethal thermal minima) and LTmax (lethal thermal maxima) were determined. Supplementation of EPA + DHA along with Se-NPs noticeably improved the thermal tolerance of the fish reared under stress (As + T) and control condition. Superoxide dismutase, glutathione-s-transferase, catalase, glutathione peroxides and LPO were enhanced by As + T, whereas EPA + DHA at 0.4 % and Se-NPs reduced the oxidative stress. Further, acetylcholine esterase was inhibited by arsenic alone and concurrent with temperature but dietary supplementation significantly enhanced the brain AChE activity. Exposure to arsenic and concurrent with a temperature significantly reduced the ATPase. Whereas supplementation of EPA + DHA at 0.4 % and Se-NPs enhanced the ATPase in liver and gill tissues. Arsenic bioaccumulation was also reduced with EPA + DHA at 0.4 % and Se-NPs. The present investigation concluded that EPA + DHA at 0.4 % and Se-NPs at 0.2 mg kg-1 diet protects the P. hypophthalmus against arsenic pollution and thermal stress.
PMID:36030006 | DOI:10.1016/j.cbpc.2022.109447