HomeIngredientRosemary oil low energy nanoemulsion: Optimization, µrheology, in silico, in vitro and Ex vivo characterization

Rosemary oil low energy nanoemulsion: Optimization, µrheology, in silico, in vitro and Ex vivo characterization

June 10, 2022

J Biomater Sci Polym Ed. 2022 Jun 10:1-20. doi: 10.1080/09205063.2022.2088527. Online ahead of print.

ABSTRACT

Acetylcholine imbalance in the human brain causes dementia-related symptoms of Alzheimer’s disease. Acetylcholinesterase (AChE) inhibitors prevent the lysis of acetylcholine in the brain and prevent dementia. Therefore, the present study aims to investigate the iIn silico and iIn vitro AChE potential of Rosemary oil and then formulate it into nanoemulsion as an adjuvant with co-administration of Donepezil (Dz) with a thought for futuristic target for Alzheimer’s diseases via the iIntranasalintranasal route. The aim of the preparing this formulation was to add the AChE inhibition effect of the rosemary oil to the effect ofthat acts as an additive effect along with Dz. The docking score of Rosemary oil components on human cholinesterase 1GQR protein was found to be >-5 Kcal/mol. In vitro AChE activity of Rosemary oil confirms the prominent IC50 of oil at 0.001 µL/min. Nanoemulsion was prepared by low energy emulsification technique using tTween 80 as surfactant and ethanol or diethylene glycol monoethyl ether as cosurfactant. Droplet size, Polydisperity index (PDI), and zeta potential of stable nanoemulsion was ∼16 nm, 0.1 and -6.05 mV for stable batch. High resolution transmission electron microscopy (HRTEM) microscopic studies depicted the spherical shape of the droplet. Micro rheology investigation of nanoemulsion clearly attributes to gel-sol-gel behaviour of formulated nanoemulsion. Thermal evaluation of nanoemulsion depicts the phase transition behaviour of ethanol based nanoemulsion at 60 °C. Ex vivo nasal ciliotoxicity and permeation studies of formulation establish the safety of ethanol based rosemary oil-loaded nanoemulsion and permeation mechanism of Dz from the nanoemulsion. In-vitro permeation studies showed more drug penetration from the nanoemulsion as compared to the plain drug. The prepared nanoemulsion were found to be stable for 3 months at 4, 25 and 45 °C of storage. This low energy preparation method can be advantageous for the preparation of scalable nanoemulsion and can be a futuristic therapeutic for Alzheimer’s disease.

PMID:35686513 | DOI:10.1080/09205063.2022.2088527

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About The Author

Patrick Blanchard MD

Meet Dr. Blanchard Dr. Blanchard’s medical practice is an embodiment of Integrative health which brings conventional and complementary approaches together in a coordinated way. Conveniently located in sunny Florida and over the internet with ValiseMD’s secure HIPPA compliant telXmed servers. Since 1994, patients with a wide range of challenging medical problems have achieved optimum health using the best of natural medicine, judiciously combined with high-tech innovations. Breakthroughs are often achieved even after patients have consulted mainstream specialists and holistic practitioners. Dr. Blanchard is founder and CEO of ValiseMD, Inc. He is board certified in Family Medicine since 1994 and awarded Fellow of the American Academy of Family Physicians in 2001. He received the ‘Teacher of the Year’ award from the University of Kansas School of Medicine at completion of his residency in Family Medicine. He completed a fellowship at Wake Forest University in the field of vascular neurosonology. He holds a medical patent in the field of Gastroenterology. He holds an unrestricted license to practice medicine and surgery in Florida. He started his medical career as a Emergency Medical Technician, then as a Paramedic and later a Medical Doctor.