HomeIngredientQuantitative HPLC method and alternative green solvents for extraction of [6]-gingerol from ginger

Quantitative HPLC method and alternative green solvents for extraction of [6]-gingerol from ginger

August 20, 2022

Pak J Pharm Sci. 2022 May;35(3):851-857.

ABSTRACT

A simple and validated HPLC method for quantitative analysis of [6]-gingerol in ginger extracts was established. The optimum HPLC conditions involved a TSK-gel ODS-80 Ts column (5 μm, 4.6 ´ 250 mm) eluted with acetonitrile and 1% aqueous acetic acid (48:52, v/v), at 30ºC, with a flow rate of 1 mL/min and a quantitative UV detection at 280 nm. A good selectivity, linearity (R² of 1.0000), accuracy (Recovery of 96.7-103.2%), intraday- and interday-precision (Relative standard deviation (RSD) of less than 2% and 5%, respectively) and sensitivity (LOD and LOD of 0.48 and 0.80 μg/mL, respectively) were obtained. A microwave-assisted extraction was used for preparation of [6]-gingerol enriched ginger extracts. Based on HPLC analysis, the concentrations of [6]-gingerol of the extracts obtained from 20% v/v glycerin in ethanol (0.45 mg/mL) as well as 20% v/v natural deep eutectic solvents (NADES) of sucrose & citric acid in ethanol (0.43 mg/mL) were not significantly different from ethanol (0.42 mg/mL). However, glycerin (20% v/v) or NADES of sucrose & citric acid (20% v/v) might be used as the alternative green solvents for preparation of [6]-gingerol extract that could be directly used for formulation of anti-nausea and vomiting pastilles.

PMID:35791487

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Patrick Blanchard MD

Meet Dr. Blanchard Dr. Blanchard’s medical practice is an embodiment of Integrative health which brings conventional and complementary approaches together in a coordinated way. Conveniently located in sunny Florida and over the internet with ValiseMD’s secure HIPPA compliant telXmed servers. Since 1994, patients with a wide range of challenging medical problems have achieved optimum health using the best of natural medicine, judiciously combined with high-tech innovations. Breakthroughs are often achieved even after patients have consulted mainstream specialists and holistic practitioners. Dr. Blanchard is founder and CEO of ValiseMD, Inc. He is board certified in Family Medicine since 1994 and awarded Fellow of the American Academy of Family Physicians in 2001. He received the ‘Teacher of the Year’ award from the University of Kansas School of Medicine at completion of his residency in Family Medicine. He completed a fellowship at Wake Forest University in the field of vascular neurosonology. He holds a medical patent in the field of Gastroenterology. He holds an unrestricted license to practice medicine and surgery in Florida. He started his medical career as a Emergency Medical Technician, then as a Paramedic and later a Medical Doctor.