Preparation and characterization of bacterial cellulose synthesized by kombucha from vinegar residue

Int J Biol Macromol. 2023 Dec 22;258(Pt 1):128939. doi: 10.1016/j.ijbiomac.2023.128939. Online ahead of print.

ABSTRACT

Bacterial cellulose (BC) has been widely applied in various fields due to its excellent physicochemical properties, but its high production cost remains a challenge. Herein, the present study aimed to utilize the hydrolysate of vinegar residue (VR) as the only medium to realize the cost-effective production of BC. The BC production was optimized by the single-factor test. The treatment of 6 % VR concentration with 3 % acid concentration at 100 °C for 1.5 h and 96 U/mL of cellulase for 4 h at 50 °C obtained a maximum reducing sugar concentration of about 32 g/L. Additionally, the VR hydrolysate treated with 3 % active carbon (AC) at 40 °C for 0.5 h achieved a total phenol removal ratio of 86 %. The yield of BC reached 2.1 g/L under the optimum conditions, which was twice compared to the standard medium. The produced BC was characterized by SEM, FT-IR, XRD, and TGA analyses, and the results indicated that the BC prepared by AC-treated VR hydrolysate had higher fiber density, higher crystallinity, and good thermal stability. Furthermore, the regenerated BC (RBC) fibers with a tensile stress of 400 MPa were prepared successfully using AmimCl solution as a solvent by dry-wet-spinning method. Overall, the VR waste could be used as an alternative carbon source for the sustainable production of BC, which could be further applied to RBC fibers preparation.

PMID:38143062 | DOI:10.1016/j.ijbiomac.2023.128939