Pre-Clinical Evaluation of Proprietary Lutein, Zeaxanthin, and Rosemary Formulation for Its Dermal Protective Activity in Male Swiss Albino Mice

Prev Nutr Food Sci. 2021 Dec 31;26(4):425-433. doi: 10.3746/pnf.2021.26.4.425.


This study aimed to evaluate the efficacy of the proprietary lutein, zeaxanthin, and rosemary formulation for its dermal protection against ultraviolet (UV) irradiated skin dehydration. A total of 48 male Swiss albino mice of 8∼12 weeks of age were divided into eight groups of 6 mice: mice in group 1 (G1) were considered the normal control, without treatment and without skin shaving; mice in G2 had their skins were shaved, but did not receive treatment; mice in G3 were the pathological control; mice in G4 were treated as standard (hyaluronic acid); mice in G5∼G8 were treated with low and high doses of 2 different test substances, respectively. Mice were anaesthetized and then depilatory was applied on the dorsal skin area (2 cm×2 cm) on alternate days, then UV/blue light irradiation was carried out for 15 min for 6 weeks. Collagen type 1 gene expression was determined via densitometric analysis, skin elasticity was assessed, and stratum corneum water contents were measured using a cutometer and corneometer. Skin hydration was assessed through transepidermal water loss, and several serum biochemical parameters (collagenase, hydroxyproline, hyaluronic acid, and ceramide levels) were determined to assess the skin moisturizing activity of the product. Images for assessing photoaging were considered between different groups on day 42. All these subjective parameters reached statistical significance (P<0.05) in groups treated with the proprietary lutein and rosemary formulation compared with the placebo-treated group. In conclusion, the proprietary lutein, zeaxanthin, and rosemary formulation showed better protection of skin subjected to UV irradiated skin dehydration.

PMID:35047439 | PMC:PMC8747954 | DOI:10.3746/pnf.2021.26.4.425