HomeIngredientGrape seed proanthocyanidins modulate the hepatic molecular clock via microRNAs

Grape seed proanthocyanidins modulate the hepatic molecular clock via microRNAs

October 3, 2022

Mol Nutr Food Res. 2022 Oct 3:e2200443. doi: 10.1002/mnfr.202200443. Online ahead of print.

ABSTRACT

SCOPE: Circadian rhythm is an endogenous and self-sustained timing system, responsible for the coordination of daily processes in 24-h timescale. It is regulated by an endogenous molecular clock, which is sensitive to external cues as light and food. We have previously shown that grape seed proanthocyanidins extract (GSPE) regulates the hepatic molecular clock. Moreover, GSPE is known to interact with some microRNAs (miRNAs). Therefore, the aim of this study was to evaluate if the activity of GSPE as modulator of hepatic clock genes could be mediated by miRNAs.

METHODS AND RESULTS: 250 mg/kg of GSPE was administered to Wistar rats before a 6-hour jet lag and sacrificed at different time points. GSPE modulated both expression of Bmal1 and miR-27b-3p in the liver. Cosinor-based analysis revealed that both Bmal1 and miR-27b-3p expression followed a circadian rhythm, a negative interaction between them, and the role of GSPE adjusting the hepatic peripheral clock via miRNA. Additionally, in vitro studies showed that Bmal1 was sensitive to GSPE (25 mg/L). However, this effect was independent of miR-27b-3p.

CONCLUSION: miRNA regulation of peripheral clocks via GSPE may be part of a complex mechanism that involves the crosstalk with the central system rather than a direct effect. This article is protected by copyright. All rights reserved.

PMID:36189890 | DOI:10.1002/mnfr.202200443

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About The Author

Patrick Blanchard MD

Meet Dr. Blanchard Dr. Blanchard’s medical practice is an embodiment of Integrative health which brings conventional and complementary approaches together in a coordinated way. Conveniently located in sunny Florida and over the internet with ValiseMD’s secure HIPPA compliant telXmed servers. Since 1994, patients with a wide range of challenging medical problems have achieved optimum health using the best of natural medicine, judiciously combined with high-tech innovations. Breakthroughs are often achieved even after patients have consulted mainstream specialists and holistic practitioners. Dr. Blanchard is founder and CEO of ValiseMD, Inc. He is board certified in Family Medicine since 1994 and awarded Fellow of the American Academy of Family Physicians in 2001. He received the ‘Teacher of the Year’ award from the University of Kansas School of Medicine at completion of his residency in Family Medicine. He completed a fellowship at Wake Forest University in the field of vascular neurosonology. He holds a medical patent in the field of Gastroenterology. He holds an unrestricted license to practice medicine and surgery in Florida. He started his medical career as a Emergency Medical Technician, then as a Paramedic and later a Medical Doctor.