Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase

Enzyme Microb Technol. 2023 Jul 27;170:110293. doi: 10.1016/j.enzmictec.2023.110293. Online ahead of print.

ABSTRACT

We present a novel approach for the enzymatic functionalization of graphene, utilizing horseradish peroxidase (HPO) and laccase (LC) from Trametes versicolor. This study demonstrates, for the first time, the covalent modification of non-homogeneous graphene with a low surface-to-volume ratio, both in solution and on solid support. Through thermogravimetry analysis, we estimate the degree of functionalization to be 11% with HPO and 4% with LC, attributed to the varying redox potentials of the enzymes. This work highlights the potential of enzymatic reactions for tailored functionalization of graphene under mild conditions.

PMID:37523883 | DOI:10.1016/j.enzmictec.2023.110293