HomeIngredientEffect of solvent composition on the extraction of proteins from hemp oil processing stream

Effect of solvent composition on the extraction of proteins from hemp oil processing stream

August 20, 2022

J Sci Food Agric. 2022 May 5. doi: 10.1002/jsfa.11979. Online ahead of print.

ABSTRACT

BACKGROUND: Hempseed meal, a by-product of hempseed oil processing stream, is a potential alternative source for food proteins. Efficient extraction of proteins from hempseed meal is challenging owing to difference in the structure and solubility of various protein fractions present in the seed. In the present study, protein was extracted from hempseed meal using four different solvents, including aqueous NaOH, KOH, NaHCO3 , and NaCl, at four different concentrations with an aim of improving the recovery of protein fractions rich in essential amino acids.

RESULTS: Extraction using alkaline solvents provided superior protein recovery (60-78%) compared with NaCl solution and control extractions (20-48% and 21%, respectively). The concentration of alkali or salt (0.25-1 M) had a minor but significant impact on the yield. Amino acid composition analysis revealed that hemp seed meal contains 24% (54.5 ± 0.19 mg/g) essential amino acids of total amino acids, and extraction with NaOH, KOH, NaHCO3 , and NaCl did not improve the selective extraction of essential amino acids compared to control experiments. SDS-PAGE analysis allowed the identification of edestin and albumin in the extracts obtained with NaHCO3 and NaCl solvents, with results further showing that the type of extraction solvent influences the protein extraction selectivity.

CONCLUSIONS: Although alkali solvents provides superior extraction yields, extraction with water resulted in extracts containing the highest proportion of proteins bearing essential amino acids. According to the results of SDS-PAGE, extraction using alkali solvents induced protein cross-linking. This article is protected by copyright. All rights reserved.

PMID:35514139 | DOI:10.1002/jsfa.11979

Tags:, ,

Related Posts

About The Author

Patrick Blanchard MD

Meet Dr. Blanchard Dr. Blanchard’s medical practice is an embodiment of Integrative health which brings conventional and complementary approaches together in a coordinated way. Conveniently located in sunny Florida and over the internet with ValiseMD’s secure HIPPA compliant telXmed servers. Since 1994, patients with a wide range of challenging medical problems have achieved optimum health using the best of natural medicine, judiciously combined with high-tech innovations. Breakthroughs are often achieved even after patients have consulted mainstream specialists and holistic practitioners. Dr. Blanchard is founder and CEO of ValiseMD, Inc. He is board certified in Family Medicine since 1994 and awarded Fellow of the American Academy of Family Physicians in 2001. He received the ‘Teacher of the Year’ award from the University of Kansas School of Medicine at completion of his residency in Family Medicine. He completed a fellowship at Wake Forest University in the field of vascular neurosonology. He holds a medical patent in the field of Gastroenterology. He holds an unrestricted license to practice medicine and surgery in Florida. He started his medical career as a Emergency Medical Technician, then as a Paramedic and later a Medical Doctor.